The Chloroplast-Located Homolog of Bacterial DNA Recombinase
نویسندگان
چکیده
منابع مشابه
Homolog ofBacterial DNA Recombinase
The cDNA fer the eh]oroplast-located homolog of bacterial RecA protein, designated rec,4=,4T, was placed.in a plasmid appropriate for in vitro transcription and translation. Translation with 35S-labe]ed Met permitted demonstration of uptake of the protein product into isolated pea chloroplasts, and processing to a mature size. Preliminary eyidence for the first amino acid was estimated from res...
متن کاملInvestigation of the secondary DNA-binding site of the bacterial recombinase RecA.
The L2 loop is a DNA-binding site of RecA protein, a recombinase from Eschericha coli. Two DNA-binding sites have been functionally defined in this protein. To determine whether the L2 loop of RecA protein is part of the primary or secondary binding site, we have constructed proteins with site-specific mutations in the loop and investigated their biological, biochemical, and DNA binding propert...
متن کاملRBF1, a plant homolog of the bacterial ribosome-binding factor RbfA, acts in processing of the chloroplast 16S ribosomal RNA.
Plastids (chloroplasts) possess 70S ribosomes that are very similar in structure and function to the ribosomes of their bacterial ancestors. While most components of the bacterial ribosome (ribosomal RNAs [rRNAs] and ribosomal proteins) are well conserved in the plastid ribosome, little is known about the factors mediating the biogenesis of plastid ribosomes. Here, we have investigated a putati...
متن کاملExpression and complementation analyses of a chloroplast-localized homolog of bacterial RecA in the moss Physcomitrella patens.
RecA protein is widespread in bacteria, and it plays a crucial role in homologous recombination. We have identified two bacterial-type recA gene homologs (PprecA1, PprecA2) in the cDNA library of the moss Physcomitrella patens. N-terminal fusion of the putative organellar targeting sequence of PpRecA2 to the green fluorescent protein (GFP) caused a targeting of PpRecA2 to the chloroplasts. Muta...
متن کاملA novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes.
We report the discovery of a novel group of highly conserved DNA sequences located within the intergenic regions of the chromosomes of Escherichia coli, Salmonella typhimurium and other bacteria. These intergenic repeat units (IRUs) are 124-127 nucleotides long and have the potential to form stable stem-loop structures. The location of these sequences within the intergenic regions is variable w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 1997
ISSN: 0032-0781,1471-9053
DOI: 10.1093/oxfordjournals.pcp.a029124